In this study, we demonstrate that L3 expression in colon tumor tissues is downregulated; L3 mRNA decrease associated with malignance progression and tumor grade and was inversely proportional to the ratio Bcl-2/Bax; L3 overexpression in 5-FU treated colon cancer cells decreased clonogenic potency, cell migration and cell viability, and stimulated apoptotic cell death by inducing late apoptosis. This evidence concerns the gene BAX and malignant colon neoplasm.