In the present study, we demonstrate the potentiation of the cytotoxic effect of 5-FU on human colon cancer cells by proapoptotic L3 and aimed to a combined therapy with the use of 5-FU along with cDNA encoding L3 in order to establish individualized combined therapy by examining L3 and p53 profiles in patient's tumors with the expectation to yield a better clinical outcomes. Here, TP53 is linked to malignant colon neoplasm.