To determine the distinct epigenotype of DEFB1 in a series of matched PCa specimens, we focused on the epialleles of the DEFB1 LCP containing the CpG 3 and CpG 4 units (Fig 2B) and compared the methylation frequencies of these two adjacent CpG sites in the microdissected samples. Here, DEFB1 is linked to posterior cortical atrophy.