After analysis, four uniquely dysregulated transcripts were selected from each SLE subgroups, CCL20 (upregulated in anti-dsDNA+), CCNA1 (upregulated in anti-ENA+), EPHB2 (upregulated in anti-dsDNA+ENA+) and ELANE (upregulated in all subsets) for further validation by TaqMan real time PCR. The gene discussed is EPHB2; the disease is systemic lupus erythematosus.