15 HCC-associated genes (p16, RASSF1A, APC, GSTP1, CDH1, p15, RUNX3, SOCS1, MGMT, SFRP1, PRDM2, DAPK1, p14, RARβ and p73) hypermethylation showed significant evidences between HCC tumor tissues and normal tissues to the risk of HCC. The gene discussed is CDKN2A; the disease is hepatocellular carcinoma.