To investigate whether the promoting effects of Gankyrin on PI3K/Akt signaling are associated with phosphoinositide metabolism, as shown in Figure 6A and 6B, Gankyrin overexpression significantly increased the levels of PI (3,4,5) P3 and PI (3,4) P2 in CRC cells, whereas knockdown of Gankyrin abrogated the aforementioned the effect. This evidence concerns the gene AKT1 and colorectal carcinoma.