MBD3 and central nervous system cancer: By fitting the ACF of MBD3-GFP with a two-component 3D diffusion model, the diffusion state of MBD3 was characterized as – the fast-moving free components (diffusion time 1-2 ms) and the slow-moving bound components (diffusion time 10-14 ms), based on which we calculated that a large proportion of MBD3-GFP (> 50%) could incorporate into the glioma epigenome (Figure 1B).