Therefore, the aim of this study was to comparatively explore the expression and activity (in terms of pro-inflammatory cytokine production) of TLR2 and TLR6 in peripheral blood mononuclear cells (PBMCs) and liver tissue from clinically characterized morbidly obese patients with different degrees of NAFLD severity. This evidence concerns the gene TLR6 and metabolic dysfunction-associated steatotic liver disease.