By performing knockdown assays and RNA-seq of the TFs HOXC6 and DLX1 in prostate cancer and annotating enhancer states in each prostate tumor sample, we found that expression of HOXC6 and DLX1 is highly linked to enhancers in the majority of prostate tumors, but they are associated with different enhancers and regulate distinct gene sets. This evidence concerns the gene DLX1 and prostate carcinoma.