This activation protocol has been now employed to (i) analyze the molecular determinants that drive allogeneic NK cell recognition of B-CLL cells and (ii) to test the susceptibility of adverse prognosis B-CLL cells, defined according to IGHV mutational status and TP53 deletion/mutation, to allogeneic activated NK cells. Here, TP53 is linked to B-cell chronic lymphocytic leukemia.