For the other top-ranked miRNAs, five have no validated targets but have many predicted targets related to breast cancer such as AKT, AKT1, CCND1, EGFR1, ERBB2, SRC, PTEN which have been used as breast cancer biomarkers for prognosis, diagnosis, drug efficacy, and disease progression [43–45]. This evidence concerns the gene PTEN and breast cancer.