In summary, our results, for the first time, illustrated the miR-199a-3p-YAP1-Jagged1-Notch signaling in HCC, in which miR-199a-3p targets YAP1 and represses the Jagged1-Notch signaling to inhibit the tumorigenesis of HCC. Here, JAG1 is linked to hepatocellular carcinoma.