HLA-C and neoplasm: There are following major parameters which need to be tested to validate a neoepitope: (a) HLA binding of the peptide, (b) Display of the neoepitope on the tumor surface on MHC molecule (can be verified either by mass spectrometry or by using a T cell raised against the neoepitope), (c) Expression of the neoantigen in the tumor cells and (d) cross reactivity which means T cells against the peptide should not recognize the wild-type peptide.