To further examine whether Drp1-mediated mitochondrial fission is necessary for the mitochondrial fragmentation and mitochondrial ROS generation observed in T-ALL cells cocultured with MSCs, we assessed the morphologies and ROS levels of mono-cultured Jurkat cells overexpressing wild-type and K38A mutant Drp1 (which inhibits mitochondrial fission).30 Immunoblotting confirmed the expression of Drp1 or Drp1 K38A vector in transfected cells (Figure 5a). This evidence concerns the gene DNM1L and acute lymphoblastic leukemia.