As shown in Figure 1b, proliferating cells were predominantly located at the bottom of crypt where Lgr5+ stem cells and transit amplifying (TA) cells reside, together referred to as the stem cell clonogen compartment.29 To assess dietary effects on stem cell proliferation, a requirement for crypt regeneration following insult, EdU+ cells in the colonic crypt were quantified following AOM-induced DNA damage. Here, LGR5 is linked to infectious otitis media.