As shown in Figure 3b (left), at 12 h post AOM exposure, n-3 PUFA and n-6 PUFA+curcumin proportionally increased MGMT expression in Lgr5+ stem cells (n-3 PUFA: slope=2.07, r=0.92 and n-6 PUFA+curcumin: slope=0.83, r=0.90), compared with n-3 PUFA and n-3 PUFA+curcumin treatment induction of MGMT in differentiated cells (GFPneg) (Figure 3b, middle). The gene discussed is LGR5; the disease is infectious otitis media.