MGMT and colonic neoplasm: Because curcumin induces MGMT protein expression in colon cancer cells, we estimated MGMT activity using quantitative immunohistochemical analysis.4 As shown in Figure 3a, MGMT expression in Lgr5+ stem cells was maximally induced by curcumin with either PUFA diet or the n-3 PUFA treatment at 24 h as compared with saline treatment.