Proteomic analysis of senescent chromatin identified the NF-κB subunit p65 as a major transcription factor that accumulates on chromatin of senescent cells.25 Components of the senescence secretome reinforce cell cycle arrest and contribute to tumor suppression by signaling and recruiting components of the immune system.41 We show the secretion of the senescence associated cytokines IL-6, GM-CSF and TNF-α; large RS cells expressed higher levels of these cytokines compared to H cells for both untreated and H2O2-treated cells. Here, TNF is linked to neoplasm.