To validate our hypothesis, our team firstly conducted a dual luciferase assay and identified that miR-142 overexpression obviously inhibited the luciferase activity of wt-3′UTR of HMGB1, however miR- 142 overexpression did not affect the luciferase activity of mut-3′UTR of HMGB1 in both cervical cancer cells (Figure 3B). The gene discussed is HMGB1; the disease is cervical cancer.