No correlation was observed between number of unmapped reads and circRNA number (R2 = 0.099) and after normalizing for unmapped reads, we still observed significant difference (p-value < 0.041) between ER+ normal-adjacent tissue and tumor samples The TN and HER2+ patients did not show significance, p-value > 0.05 (Supplementary Figure S1) and combining all subtypes (89 pairs) yielded p-value < 0.1. This evidence concerns the gene ESR1 and neoplasm.