After treatments, we analyzed the levels of TRPV1 transcript by western blot analysis, and the results evidenced a reduced expression of TRPV1 proteins in both glioma cell lines, mainly in U87 cells (Figure 3B), suggesting that IFN-γ was able to negatively regulate GAIT-TRPV1 variants translation, with levels of TRPV1 protein likely sustained by the TRPVv1 expression that does not contain a GAIT element in its 5'UTR region. The gene discussed is TRPV1; the disease is glioma.