We observed that the expression of cell cycle-related factors (PCNA, CyclinD1 and CDC25A), the expression of metastasis up-related factors (KPNA2, MELK and KIF2C) and RHOA-REG pathway-related factors (RHOA, ROCK1 and ARHGAP5) was lower, while DLC1 was higher in lung cancer cells with TRIM65 knockdown. The gene discussed is RHOA; the disease is lung cancer.