SUMO1 and colorectal carcinoma: To confirm MAFB was also modified by SUMO1 in CRC cells, western blotting was performed using antibodies against MAFB (rabbit polyclonal antibody) and SUMO1 to detect the MAFB-SUMO1 conjugates immunoprecipitated via antibodies against MAFB (goat polyclonal antibody) from SW1116 or HCT116 cell lysates.