The analysis performed for different neuroblastoma stages showed a significant association between decreased expression of miR-181c and a worse overall survival of stage 4 neuroblastoma cases with no MYCN amplification (χ2 = 7.17, df = 1, p-value = 7.4e-03, n = 116), but not with amplified MYCN (χ2 = 1.7, df = 1, p-value = 0.192, n = 65) (Supplementary Figure S5). This evidence concerns the gene MYCN and neuroblastoma.