Finally, we developed based on the results of differential expression analysis and characteristic immunoprofiles (S2 Fig) a simplified diagnostic immunohistochemical panel including eight highly discriminant markers between the immunohistochemical tumor types (Table 1): CK19, CK20, MUC2, MUC5AC, CA19-9, monoclonal CEA, CA125 and SMAD4. This evidence concerns the gene SMAD4 and neoplasm.