MITF and melanoma: Finally, by flow cytometry we quantified cell cycle distribution in BrdU and/or propidium iodide stained cell populations (Supplementary Fig. 2f,g), demonstrating that, in melanoma cells, FANC and MiTF depletion with specific siRNAs triggers a similar G0/G1 phase accumulation together with a corresponding reduction in the frequency of S-phase cells, as evaluated 72 hours post-siRNA transfection.