NKX2-1 and Anxiety: Conversely, Tg(Nkx2.1-Cre);Syngap1flox/+ showed normal levels of locomotion and anxiety-like behaviour compared with Syngap1flox/+mice (Fig. 7a,b; for open field: 45±5 m for Syngap1flox/+ versus 51±6 m for Tg(Nkx2.1-Cre);Syngap1flox/+, Mann–Whitney rank sum test, P=0.503; for elevated plus maze: 15±3% for Syngap1flox/+ versus 21±3% Tg(Nkx2.1-Cre);Syngap1flox/+, Student's t-test, P=0.136).