Six weeks after AAV-mediated miRNA delivery, wild-type mice with hepatocyte-specific TSC22D4 deficiency (Supplementary Fig. 2a) showed no change in fasting blood glucose levels (Fig. 2a) but displayed significantly reduced levels of serum insulin (Fig. 2b) and an improved homeostasis model of assessment-insulin resistance (HOMA-IR) index as compared with control littermates (Fig. 2c). Here, TSC22D4 is linked to Insulin resistance.