B16F10 melanoma cells were treated with various doses (0.1, 1, and 10 ng/ml) of GH for 3 h (Fig. 4a), and then at various time intervals (0, 1, 3 and 6 h) at GH 10 ng/ml (Fig. 4b; F = 22.362, P < 0.05). The gene discussed is GH1; the disease is melanoma.