We have previously shown that insertion of CD8 epitopes TRP2 (aa180-188) and CD4 epitopes from gp100 (aa44-59 and 174-190) into human IgG1 antibody DNA vaccine (SCIB1) induces high frequency and avidity CD8 and CD4 T cell responses in mice [17] and melanoma patients [19]. Here, PMEL is linked to melanoma.