SIRT6 and hepatocellular carcinoma: The percentages of SIRT6-depleted HCC cells positively stained with SA-ß-gal were 29.1 ± 4.9% (shSIRT6-1) and 16.2 ± 8.4% (shSIRT6-3) Hep3B cells and 29.3 ± 5.2% (shSIRT6-1) and 17.1 ± 6.3% (shSIRT6-1) Huh-7 cells, while that of control HCC cells positively stained with SA-ß-gal was less than 1.0% Hep3B cells and 6.1% Huh-7 cells (Fig 3B).