MRC1 and neoplasm: Flow cytometry showed that these macrophages also highly expressed CD68 and CD206, which supported the previous founding that tumor cells has the ability to drive macrophages to M2 phenotype.[17, 18] To further determine whether these cocultured tumor cells acted as mural-like cells, we tested the protein expression of smooth muscle α-actin (SMA, specific marker of mural cells) and VE-cadherin (specific marker of vascular endothelial cells) in our target cells.