We used a high cholesterol (HC-) diet as previously described by Hartvigsen and colleagues,10 to induce hypercholesterolemia in Ldlr-/- mice, closely reflecting the lipid profile of an FH patient without inducing other concomitant metabolic disorders, such as hypertriglyceridaemia, which may independently drive atherosclerosis. This evidence concerns the gene LDLR and Other metabolic disease.