The reduced expression of the epithelial marker E-cadherin (CDH1), with the concurrent increase in the expression of the mesenchymal markers fibronectin (FN1) or vimentin (VIM) in the HCT116 cell line, as well as in two other colorectal cancer cell lines Caco2 and HT29 (Fig. 3A–C, Supplementary Fig. S2A and B) confirmed the induction of epithelial-mesenchymal transition by D-2HG across colorectal cancer cells with different genetic background (Supplementary Table S1). This evidence concerns the gene CDH1 and colorectal cancer.