Expressions of 5-LOX and Bcl-2 proteins were decreased significantly; expressions of Bax and active caspase-3 were increased significantly in KuA treated U251cells, WJ1 cells (Fig. 6A–D, p < 0.01) and tumor tissues (Fig. 6I,J, p < 0.01); the expressions of C/EBPβ, N-cadherin, vimentin, twist and snail+slug were decreased significantly, while the expression of E-cadherin was increased significantly in KuA treated U251cells, WJ1 cells (Fig. 6E–H, p < 0.01) and tumor tissues (Fig. 6K,L, p < 0.01) compared with the controls. This evidence concerns the gene TWIST1 and neoplasm.