In epithelial to mesenchymal transition, E-cadherin is a marker of epithelial phenotype, while, N-cadherin and vimentin are the markers for mesenchymal phenotype39; Twist, slug and snail are the master regulators of the epithelial-mesenchymal transition40, 41, they worked coordinately in regulation of GBM cell migration, invasion and metastasis through induction of EMT. This evidence concerns the gene TWIST1 and glioblastoma.