We overexpressed eGFP-Mena, eGFP-MenaINV and eGFP-Mena11a in MDA-MB-23143 and MTLn344 cells at approximately 4-fold the level of endogenous Mena expression to simulate the level of spontaneous MenaINV expression seen previously in the invasive subpopulations of breast carcinoma cells in vivo in two different mouse mammary tumors40. Here, EGFR is linked to breast carcinoma.