ENAH and breast carcinoma: Using both rat MTLn3 and human MDA-MB-231 breast carcinoma cell lines stably expressing eGFP-Mena, eGFP-MenaINV and eGFP-Mena11a, we found that all of these Mena isoforms co-localize with cortactin and Tks5 at invadopodium precursors (Fig. 1A; Supplementary Fig. S1).