By comparing the methylation level between stage III and stage IV of HCC, we found that in stage IV, 8 genes have differential methylation, i.e. TRAF2, IL1R1, MAP3K2, GNA12, IL1B, CNTFR, IL7R, and STAT3 as shown in Figure 5. The gene discussed is IL1R1; the disease is hepatocellular carcinoma.