By comparing the methylation level between stage III and stage IV of HCC as shown in Figure 5, we found that 8 genes expressed in stage III have differential methylation, i.e. TRAF2, HRAS, PDGFB, NFKB2, DAXX, MYC, GDF6, and BMPR1B. The gene discussed is GDF6; the disease is hepatocellular carcinoma.