In addition, 8 genes were differentially methylated between stage II and III of HCC (TRAF2, TNFRSF1A, HRAS, PDGFB, NFKB1, DAXX and MYC in the MAPK pathway, and MYC in TGF-β pathway). The gene discussed is NFKB1; the disease is hepatocellular carcinoma.