To test this hypothesis, we overexpressed wild type TRIM71 (TRIM71-WT) and its RING finger mutant (TRIM71-CA) in the NSCLC cell line NCI-H1299, which expresses high levels of Lin28B and HMGA2, and in the NCI-H460 cell line, which does not express either protein, using lentivirus transduction. The gene discussed is TRIM71; the disease is non-small cell lung carcinoma.