Although it has been reported that TWEAK promotes glioblastoma cell [34, 36–38] and ovarian cancer cell [35] migration in modified Boyden chamber or radial cell migration assays, we found that sTWEAK overexpression in B16 melanoma cells had no significant effect on cell motility using the scratch wound migration assay. The gene discussed is TNFSF12; the disease is ovarian carcinoma.