Correlating with our previous results using lung tissues, the median Em/Ad increased in the EBCs of LC patients (n = 48) to 1.32 (P = 2.26E‐16) for GATA6 and to 1.13 (P = 1.6E‐17) for NKX2‐1. Here, NKX2-1 is linked to laryngotracheoesophageal cleft.