APPL1 and hepatocellular carcinoma: Using western blot to analyze a series of paired human tumors and adjacent non-tumor tissues for the expression pattern of APPL1, we found that APPL1 was highly expressed in human hepatocellular carcinoma (HCC) and triple-positive breast cancer (TPBC) when compared with their matched non-tumor counterparts (Fig 1A and 1D), suggesting a positive correlation of APPL1 with tumorigenesis.