Next, we tested whether inducible knockdown of RPN5, ERN1, and Bap60 can also regulate Toll- and IMD-mediated NF-κB activation by measuring drosomycin and diptericin expression, respectively, in Gram-positive and Gram-negative bacterial infection conditions in the C654ts-Gal4 RNAi flies. This evidence concerns the gene ERN1 and gram-negative bacterial infections.