Besides, a decreased risk of PCa was observed among subgroups age >68 years, a BMI of <25 kg/m2, with hypertension, without diabetes mellitus and without cardiovascular disease for patients carrying MTHFR rs1801133 TT genotype; and an increased risk of PCa was observed among subgroups with cardiovascular disease with lymph node involvement and those carrying the MTHFR rs1801131 CC genotype, further homogeneity tests didn’t support the differences in PCa risk found between these strata. Here, MTHFR is linked to diabetes mellitus.