MYBL2, CENPF, CDKN3, and CDCA3 were upregulated in EC tissues (Figure 2). MYBL2 is frequently amplified in gastroesophageal cancer cell lines and Barrett's adenocarcinoma [37, 38]. CENPF is frequently amplified in region around 1q32-q41 and is overexpressed in ESCC cell line [39]. This evidence concerns the gene CENPF and esophageal squamous cell carcinoma.