Nevertheless, to test the potential effect of NKG2D expression on survival of leukemia mice receiving IL pre-activation and re-stimulation, Rag1KO B6 mice were divided into two groups, one group of mice received anti-NKG2D antibody for neutralization, another group of mice received IgG1 as control, while both groups of mice were given IL pre-activation and re-stimulation as described above. This evidence concerns the gene KLRK1 and leukemia.