Next, we examined EWSAT1 expression in NPC cell lines, and found that EWSAT1 was over-expressed in CNE-2, C666-1, HNE-1, CNE-1, SUNE-1, and HONE-1 cells, compared with that of in NP69 cells (a normal NP cell lines) (Fig. 1B). This evidence concerns the gene EWSAT1 and nasopharyngeal carcinoma.