And then, trypan blue staining, colony formation as well as CCK8 assay were conducted to explore the role of EWSAT1 on NPC cell growth, and results demonstrated silence of EWSAT1 induced a reduction in the cell growth of CNE-1 and SUNE-1 cells than that of in their blank counterparts (Fig. 2A-C, D and F). The gene discussed is EWSAT1; the disease is nasopharyngeal carcinoma.