The minimum mandatory tests are (recommendation grade A): physical examination, complete blood count, serum creatinine, electrolytes and liver enzymes, pre- and post-orchiectomy serum tumor markers [alpha-fetoprotein (AFP), beta-human chorionic gonadotropin (BHCG) and lactate dehydrogenase (LDH)], chest (mandatory in non-seminoma), abdomen, and pelvic CT scan. The gene discussed is AFP; the disease is seminoma.