ACAN and amyotrophic lateral sclerosis: Several important DEGs were identified, including Tumor necrosis factor (TNF), Endothelin 1 (EDN1), Angiotensin (AGT), Apolipoprotein E (APOE), Vitronectin (VTN), Von Willebrand factor (VWF), Thrombospondin receptor (CD36), TNF Receptor Superfamily Member 5 (CD40), Integrin, Alpha 3 (ITGA3), Integrin, Alpha 7 (ITGA7), Transforming growth factor beta 2 (TGFB2), Aggrecan (ACAN),Wingless-Type MMTV Integration site family, member 5A (WNT5A), Neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) and Zinc finger protein GLI1 (GLI1) which were involved in the biological processes related to ALS.