In contrast, Shh null mice, which display severe cases of persistent cloaca, and Gli2 as well as Gli3 null mice, which display imperforate anus with recto-urethral fistula and anal stenosis respectively, reveal the importance of hedgehog signaling in anorectal and urogenital development in both male and female [16]. The gene discussed is GLI2; the disease is imperforate anus.