PVT1 and osteosarcoma: Moreover, we found that the mRNA expression level of PVT1 was upregulated in osteosarcoma cell lines (KHOS, 143b, LM7, U2OS, and MG-63) compared with a normal osteoblast cell line NHost (P < 0.05), and PVT1 expression was higher in U2OS and MG-63 cells than other osteosarcoma cells (Figure 1B).