TOLLIP and amyotrophic lateral sclerosis: At passages 4 and 8, TOLLIP was overexpressed in patients with ALS relative to controls (2.52-fold, p<0.001; 1.17-fold, p = 0.011); however, its expression decreased with increasing passages and the expression of TOLLIP in patients with ALS was lower than that in controls at passages 12 (0.85-fold; Fig 5).