We speculated that this phenomenon may be resulted by two reasons: 1) miR-22 only specifically target to AKT3 mRNA, and which only occupies a minor part of total mRNA expression of AKT (2.71% ± 3.33%, data not shown); 2) Overexpression of YWHAZ could enhance AKT phosphorylation in cancer cells via binding the p85 regulatory subunit of PI3K [46]. The gene discussed is AKT1; the disease is cancer.