The effectiveness of the treatment on PD was evaluated by levels of tyrosine hydroxylase (TH), dopamine (DA), dihydroxyphenyl acetic acid (DOPAC), homovanillic acid (HVA), superoxide dismutase (SOD), glutathione (GSH), and brain-derived neurotrophic factor (BDNF) (Additional file 4). The gene discussed is BDNF; the disease is Parkinson disease.